70 DAY ANTIBODY PRODUCTION PACKAGE - IN RABBITS

This protocol replaces the Traditional Antibody Production Protocol that we offered. We have kept the the immunization and bleeding schedule the same. Please keep in mind that antigen in gel can only be injected subcutaneous. If your antigen is in a gel, we can still follow this protocol. We make all injections subcutaneous. No price adjustment is offered for changing the route of immunization. Alternatively, the 91 day protocol can be selected.

Day 0 - Preimmune Bleed, 3 to 4ml of sera. Preimmune bleed is stored until the first immune bleed is sent.

Inject 200ug intradermal in Complete Freund's Adjuvant (FCA). Antigen supply should be 1mg/ml or higher.

DAY 14 - Inject 100ug intradermal in Incomplete Freund's Adjuvant (IFA)

DAY 28 - Inject 100ug subcutaneous with IFA.

DAY 42 - Small Bleed (3 to 4ml sera) - This bleed will be sent to you along with the preimmune bleed.

DAY 56 - Inject 50ug subcutaneous with IFA

DAY 70 - SELECT ONE OF THE THREE OPTIONS BELOW:

For CLOSED projects - Large bleed at day 70 - rabbit is then euthanized / discard (lowest cost). The bleed is then sent to you.

For CLOSED projects - The rabbit is exsanguinated (final bleed) at day 70. The exsanguination sera is then sent to you.

For OPEN projects - Small Bleed - This bleed is then sent to you.

AFTER DAY 70 FOR OPEN PROJECTS - You may select one of the project extensions below. If you do not specify a project extension, we will automatically begin Extension A. Keep in mind that all procedures and maintenance after day 70 will be charged according to the Custom Polyclonal Antibody Production Price Schedule.

EXTENSION A: We will boost every four weeks with 25-50ug of antigen subcutaneous with IFA. We will do a large bleed two weeks after each boost and ship. We will continue to boost and bleed every four weeks until you contact us.

EXTENSION B: When the antisera contains sufficient antibody titer and large volumes of sera are needed, we can start production bleeds. We do production bleeds every seven days. We will continue with production bleeds until you contact us or the health of the rabbit requires us to stop production bleeds. The volume of serum collected for large or production bleeds depends on the weight and the general health of the Rabbit. We generally take 25-45ml of blood yielding 13 to 20ml of serum. During this time, we boost the rabbits every four weeks with 25-50ug of antigen subcutaneous with IFA. You may also request that we not do any boosts after the initial immunization scheme outlined above since some antigens and some animals may respond negatively to boosts during the period of peak antibody activity. Boosts may be required only when titer drops. It is up to the investigator to decide and let us know if we should discontinue boosts as our standard schedule provides for boosts every 28 days. Shipping for production bleeds is every four weeks unless you request otherwise.

EXTENSION C: Some investigators prefer to "wait and see." after day 70. Extension C is hold without boosting and bleeding. We will hold until you tell us to do something else.

TERMINATION: When you have collected enough antisera, you will need to contact us to terminate the project. You may request that we terminate by:
1. Exsanguination - termination with sera - the rabbit is anesthetized and the terminal bleed is taken. For best results, rabbits should be boosted 10 to 21 days before exsanguination.
2. Discard - termination without sera - the rabbit is humanely euthanized.

When requesting termination, you must confirm the species and animal number. Written confirmation by email or fax may be requested for terminal procedures.

CUSTOM EXTENSION: You may design your own project extension. Let us know what we can do for you!

70 DAY ANTIBODY PRODUCTION PACKAGE - IN GUINEA PIGS

This protocol replaces the Traditional Antibody Production Protocol that we offered. We have kept the the immunization and bleeding schedule the same. Please keep in mind that antigen in gel can not be injected intramuscular. If your antigen is in a gel, we can still follow this protocol, but all injections will be subcutaneous or you can select the 91 day protocol.

DAY 0 - Preimmune Bleed, 3 to 4ml of sera. Preimmune bleed is stored until the first immune bleed is sent.

Inject 200ug intramuscular in Complete Freund's Adjuvant (FCA). Antigen supply should be 1mg/ml or higher.

DAY 14 - Inject 100ug intramuscular in Incomplete Freund's Adjuvant (IFA)

DAY 28 - Inject 100ug intramuscular with IFA.

DAY 42 - Bleed, 3 to 5ml sera - This bleed will be sent to you along with the preimmune bleed.

DAY 56 - Inject 50ug intramuscular with IFA

DAY 70 - SELECT ONE OF THE THREE OPTIONS BELOW:

For CLOSED projects - Bleed at day 70 - guinea pig is then euthanized / discard (lowest cost). The bleed is then sent to you.

For CLOSED projects - The guinea pig is exsanguinated (final bleed) at day 70. The exsanguination sera is then sent to you.

For OPEN projects - Bleed - This bleed is then sent to you.

AFTER DAY 70 FOR OPEN PROJECTS - You may select one of the project extensions below. If you do not specify a project extension, we will automatically begin Extension A. Keep in mind that all procedures and maintenance after day 70 will be charged according to the Custom Polyclonal Antibody Production Price Schedule.

EXTENSION A: We will boost every four weeks with 25-50ug of antigen intramuscular with IFA. We will do a large bleed two weeks after each boost and ship. We will continue to boost and bleed every four weeks until you contact us.

EXTENSION B: When the antisera contains sufficient antibody titer and large volumes of sera are needed, we can start production bleeds. We do production bleeds every seven days. We will continue with production bleeds until you contact us or the health of the guinea pig requires us to stop production bleeds. The volume of serum collected for large or production bleeds depends on the weight and the general health of the guinea pig. We generally take 8ml of blood yielding 3 to 4ml of serum. During this time, we boost the guinea pigs every four weeks with 25-50ug of antigen intramuscular with IFA. You may also request that we not do any boosts after the initial immunization scheme outlined above since some antigens and some animals may respond negatively to boosts during the period of peak antibody activity. Boosts may be required only when titer drops. It is up to the investigator to decide and let us know if we should discontinue boosts as our standard schedule provides for boosts every 28 days. Shipping for production bleeds is every four weeks unless you request otherwise.

EXTENSION C: Some investigators prefer to "wait and see." after day 70. Extension C is hold without boosting and bleeding. We will hold until you tell us to do something else.

TERMINATION: When you have collected enough antisera, you will need to contact us to terminate the project. You may request that we terminate by:
1. Exsanguination - termination with sera - the guinea pig is anesthetized and the terminal bleed is taken. For best results, guinea pigs should be boosted 10 to 21 days before exsanguination.
2. Discard - termination without sera - the guinea pig is humanely euthanized.

When requesting termination, you must confirm the species and animal number. Written confirmation by email may be requested for terminal procedures.

CUSTOM EXTENSION: You may design your own project extension. Let us know what we can do for you!

70 DAY ANTIBODY PRODUCTION PACKAGE - IN CHICKENS

This protocol replaces the Traditional Antibody Production Protocol that we offered. We have kept the the immunization and bleeding schedule the same. Please keep in mind that antigen in gel can not be injected intramuscular. If your antigen is in a gel, we can still follow this protocol, but all injections will be subcutaneous or you can select the 91 day protocol.

DAY 0 - Preimmune Bleed, about 5ml of sera. Preimmune bleed is stored until the first immune bleed is sent.

Inject 200ug subcutaneous in Complete Freund's Adjuvant (FCA). Antigen supply should be 1mg/ml or higher.

DAY 14 - Inject 100ug subcutaneous in Incomplete Freund's Adjuvant (IFA)

DAY 28 - Inject 100ug subcutaneous with IFA.

DAY 42 - Bleed, about 5ml sera - This bleed will be sent to you along with the preimmune bleed.

DAY 60 - Start Collect Eggs - This package includes six immune eggs

DAY 70 - SELECT ONE OF THE THREE OPTIONS BELOW:

For CLOSED projects - Bleed at day 70 - chicken is then euthanized / discard (lowest cost). The bleed and collected eggs are then sent to you.

For CLOSED projects - The chicken is exsanguinated (final bleed) at day 70. The exsanguination sera and collected eggs are then sent to you.

For OPEN projects - This bleed is sent to you, along with the collected eggs.

AFTER DAY 70 FOR OPEN PROJECTS - You may select one of the project extensions below. If you do not specify a project extension, we will automatically begin Extension A. Keep in mind that all procedures and maintenance after day 70 will be charged according to the Custom Polyclonal Antibody Production Price Schedule.

EXTENSION A: We will boost every four weeks with 25-50ug of antigen subcutaneous with IFA. We will do a large bleed two weeks after each boost and ship. We will continue to boost and bleed every four weeks until you contact us.

EXTENSION B: When the antisera contains sufficient antibody titer and large volumes of sera are needed, we can start production bleeds. We do production bleeds every seven days. We will continue with production bleeds until you contact us or the health of the chicken requires us to stop production bleeds. The volume of serum collected for large or production bleeds depends on the weight and the general health of the guinea pig. We generally take 10 to 15ml of blood yielding about 5ml of serum. During this time, we boost the chickens every four weeks with 25-50ug of antigen subcutaneous with IFA. You may also request that we not do any boosts after the initial immunization scheme outlined above since some antigens and some animals may respond negatively to boosts during the period of peak antibody activity. Boosts may be required only when titer drops. It is up to the investigator to decide and let us know if we should discontinue boosts as our standard schedule provides for boosts every 28 days. Shipping for production bleeds is every four weeks unless you request otherwise.

EXTENSION C: Some investigators prefer to "wait and see." after day 70. Extension C is hold without boosting and bleeding. We will hold until you tell us to do something else.

EGGS: Immune eggs can be collected anytime during the protocol. The investigator must request that eggs be collected during any of the project extension phases. Keep in mind that there is a charge for collecting, storing, and separating the yolks for shipment. The egg collection fee can be waived if PRF&L does any egg purification service on the collected eggs.

TERMINATION: When you have collected enough antisera, you will need to contact us to terminate the project. You may request that we terminate by:
1. Exsanguination - termination with sera - the chicken is anesthetized and the terminal bleed is taken. For best results, chickens should be boosted 10 to 21 days before exsanguination.
2. Discard - termination without sera - the chicken is humanely euthanized.

When requesting termination, you must confirm the species and animal number. Written confirmation by email may be requested for terminal procedures.

CUSTOM EXTENSION: You may design your own project extension. Let us know what we can do for you!

70 DAY ANTIBODY PRODUCTION PACKAGE - RATS AND MICE

This protocol replaces the Traditional Antibody Production Protocol that we offered. We have kept the the immunization and bleeding schedule the same. Please keep in mind that antigen in gel can not be injected intraperitoneal. If your antigen is in a gel, we can still follow this protocol, but all injections will be subcutaneous or you can select the 91 day protocol.

DAY 0 - Preimmune Bleed, about 0.1ml of sera. Preimmune bleed is stored until the first immune bleed is sent.

Inject 50 to 100ug intraperitoneal in Complete Freund's Adjuvant (FCA). Antigen supply should be 1mg/ml or higher.

DAY 14 - Inject 25 to 50ug intraperitoneal in Incomplete Freund's Adjuvant (IFA)

DAY 28 - Inject 25 to 50ug intraperitoneal with IFA.

DAY 42 - Bleed. About 0.1ml sera for mice and 0.5ml for rats - This bleed will be sent to you along with the preimmune bleed.

DAY 56 - Inject 10 to 25ug intraperitoneal with IFA

DAY 70 - SELECT ONE OF THE THREE OPTIONS BELOW:

For CLOSED projects - Bleed at day 70 - Rat or Mouse is then euthanized / discard (lowest cost). The bleed is then sent to you.

For CLOSED projects - The Rat or Mouse is exsanguinated (final bleed) at day 70. The exsanguination sera is then sent to you.

For OPEN projects - Bleed - This bleed is then sent to you.

AFTER DAY 70 FOR OPEN PROJECTS - You may select one of the project extensions below. If you do not specify a project extension, we will automatically begin Extension A. Keep in mind that all procedures and maintenance after day 70 will be charged according to the Custom Polyclonal Antibody Production Price Schedule.

EXTENSION A: We will boost every four weeks with 10-25ug of antigen intraperitoneal with IFA. We will do a large bleed two weeks after each boost and ship. We will continue to boost and bleed every four weeks until you contact us.

EXTENSION B: When the antisera contains sufficient antibody titer and large volumes of sera are needed, we can start production bleeds. We do production bleeds every seven days. We will continue with production bleeds until you contact us or the health of the animal requires us to stop production bleeds. The volume of serum collected for large or production bleeds depends on the weight and the general health of the rat or mouse. Generally, bleed volumes will not vary from the bleeds previously drawn. During this time, we boost the animal every four weeks with 10-25ug of antigen intraperitoneal with IFA. You may also request that we not do any boosts after the initial immunization scheme outlined above since some antigens and some animals may respond negatively to boosts during the period of peak antibody activity. Boosts may be required only when titer drops. It is up to the investigator to decide and let us know if we should discontinue boosts as our standard schedule provides for boosts every 28 days. Shipping for production bleeds is every four weeks unless you request otherwise. Please keep in mind, unlike our other species, rats and mice do not tolerate many cycles of production bleeds.

EXTENSION C: Some investigators prefer to "wait and see." after day 91. Extension C is hold without boosting and bleeding. We will hold until you tell us to do something else.

TERMINATION: When you have collected enough antisera, you will need to contact us to terminate the project. You may request that we terminate by:
1. Exsanguination - termination with sera - the rat or mouse is anesthetized and the terminal bleed is taken. For best results, rats and mice should be boosted 10 to 21 days before exsanguination.
2. Discard - termination without sera - the rat or mouse is humanely euthanized.

When requesting termination, you must confirm the species and animal number. Written confirmation by email may be requested for terminal procedures.

SPLEEN HARVEST: At the time of exsanguination, we are able to harvest the spleens. BALB/c mice and Long Evans rats are used for antibody production. Please specify the conditions of which you want the spleen. Additional charges apply for spleen harvests.

CUSTOM EXTENSION: You may design your own project extension. Let us know what we can do for you!

DAY 0 - Preimmune Bleed, about 60ml of sera. Preimmune bleed is stored until the first immune bleed is sent. Other preimmune bleed options are available for goats and sheep.

Inject 200ug to 500ug intradermal in Complete Freund's Adjuvant (FCA). Antigen supply should be 0.8mg/ml or higher.

DAY 14 - Inject 100ug intradermal in Incomplete Freund's Adjuvant (IFA)

DAY 28 - Inject 100ug intramuscular with IFA.

DAY 42 - Small Bleed, about 60ml of sera - This bleed will be sent to you along with the preimmune bleed.

DAY 56 - Inject 50ug intramuscular with IFA

DAY 70 - SELECT ONE OF THE THREE OPTIONS BELOW:

For CLOSED projects - Large bleed at day 70 - Goat or Sheep is then euthanized / discard (lowest cost). The bleed is then sent to you.

For CLOSED projects - The Goat or Sheep is exsanguinated (final bleed) at day 70. The exsanguination sera is then sent to you.

For OPEN projects - Small Bleed - This bleed is then sent to you.

AFTER DAY 70 FOR OPEN PROJECTS - You may select one of the project extensions below. If you do not specify a project extension, we will automatically begin Extension A. Keep in mind that all procedures and maintenance after day 70 will be charged according to the Custom Polyclonal Antibody Production Price Schedule.

EXTENSION A: We will boost every four weeks with 50ug of antigen intramuscular with IFA. We will do a large bleed two weeks after each boost and ship. We will continue to boost and bleed every four weeks until you contact us.

EXTENSION B: When the antisera contains sufficient antibody titer and large volumes of sera are needed, we can start production bleeds. We do production bleeds every seven days. We will continue with production bleeds until you contact us or the health of the goat or sheep requires us to stop production bleeds. The volume of serum collected for large or production bleeds depends on the weight and the general health of the animal. We generally take 200 to 500ml of blood yielding 100 to 250ml of serum. During this time, we boost the goats and sheep every four weeks with 50ug of antigen intramuscular with IFA. You may also request that we not do any boosts after the initial immunization scheme outlined above since some antigens and some animals may respond negatively to boosts during the period of peak antibody activity. Boosts may be required only when titer drops. It is up to the investigator to decide and let us know if we should discontinue boosts as our standard schedule provides for boosts every 28 days. Shipping for production bleeds is every four weeks unless you request otherwise.

EXTENSION C: Some investigators prefer to "wait and see." after day 70. Extension C is hold without boosting and bleeding. We will hold until you tell us to do something else.

TERMINATION: When you have collected enough antisera, you will need to contact us to terminate the project. You may request that we terminate by:
1. Exsanguination - termination with sera - the goat or sheep is anesthetized and the terminal bleed is taken. For best results, goat and sheep should be boosted 10 to 21 days before exsanguination.
2. Discard - termination without sera - the goat or sheep is humanely euthanized.

When requesting termination, you must confirm the species and animal number. Written confirmation by email may be requested for terminal procedures.

CUSTOM EXTENSION: You may design your own project extension. Let us know what we can do for you!