Monoclonal Antibody Production by In-Vivo Methods (Ascites Production)
Price per cell line, includes cell line preparation (when provided with Mycoplasma free live cell line) for immunization into 5 Balb/c Mice ( or another hybridoma specific strain maybe additional), all necessary procedures and husbandry for a typical production run which is based on removing ascites fluid a maximum of 3 times per mouse followed by euthanasia and disposal. Yields differ greatly between cell lines but if the line is a reasonable producer, 3 to 10 ml per mouse can be anticipated.
$ 699.00
Each additional mouse added to production run by passing on the line from mouse
to mouse. $99.00 ea
Protein G Purification of Ascites Fluid
(~ yield 0.5 - 2mg or > / ml of neat ascites fluid)
25 ml or less .. $375
up to 50 ml .. $500
up to 100 ml .. $725
Custom Monoclonal Antibody Production
by In-Vitro Methods . . . . . . . . . . . . . . . . . . . . . . . . . . $1,450
Yield of approximately 50 - 200mg per batch The customer's cell line is amplified using three liter tissue culture polyethylene bags. Monoclonal antibodies are isolated using a protein G column. Available only for IgG producing lines.
Other protocols are available and the success with a certain protocol is
dependent on the cell line. Please consult with our technical staff prior
to starting an in vitro project.
Initial Cell Culture - When lines are supplied to us frozen.
We will culture, expand, and assess quality prior to ascites or in vitro
Production (ELISA method), per line . . . . . . . . . . . . . . . . $450
Amplification & Cryopreservation 10 vials @ 1 to 2 million cells
per vial per line and storage for the first year in liquid nitrogen . $450
Each additional year . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . $250
Mycoplasma Testing
First line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . $350
Each additional lines to be tested at the same time . . . . . . . . . .$150
Isotyping
First line / Antibody . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . $150
Each additional lines to be tested at the same time . . . . . . . . . . $75
Quantitation of Mouse IgG in hybridoma supernatant or ascites
First sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . $250
Each additional sample at the same time . . . . . . . . . . . . . . . . . .$75
Conjugation to Biotin or other targets
Conjugation of up to 10mg of antibody to biotin or other target . . $450
Conjugation of same antigen to an additional carrier . . . . . $250
AFFINITY PURIFICATION OF MONOCLONAL ANTIBODIES
Covalently link 1 - 2mg of antigen to column through amino groups.
$750 for 30ml sera or less
$1000 for 31-100ml sera
please inquire about higher volumes
ANTIBODY TITRATION BY ELISA
TITRATION ASSAY IN 96 WELL MICROPLATE ASSAY PERFORMED IN DUPLICATE
10 - 20ug antigen needed per plate for coating
Protein concentration must be provided
Need to know buffer conditions (any detergent?)
$95 for first sample
$55 for each additional sample to same antigen
WESTERN BLOT ASSAY
FOUR LANES ASSAYED
100ug antigen needed per lane
Protein concentration must be provided
Need to know buffer conditions (any detergent?)
Approximate molecular weight of protein of interest
$230 for first four lanes
$95 for each additional four lanes
SDS POLYACRYLAMIDE GEL ELECTROPHORESIS
OF ANTIGENS
FOUR LANES ASSAYED
SAMPLE RUN UNDER REDUCING CONDITIONS
COOMASSIE BLUE STAINED GELS
100ug antigen needed per lane
Protein concentration and approximate size must be provided
Need to know buffer conditions (any detergent?)
$185 for first four lanes
$75 for each additional four lanes
