PHASE A.
Immunization of BALB/c Mice and Serum Screening
(approximately 6-8 weeks)
Eight mice are immunized with antigen provided by customer. Antigen conjugation and purification services are available for an additional charge. At day 35 mice are test bled and screened for titer by ELISA.

PHASE B.
Fusion and screening of Hybridoma Supernatant
(approximately an additional 8-10 weeks)
Upon successful completion of Phase A, selected mice (two) will be euthanized, spleens will be harvested and a fusion procedure will be performed. Viable supernatants will be expanded in multiple well plates. After expansion, all wells containing fusions will be screened by ELISA (10 per spleen) using previously supplied antigens. Subsequent screening by Western Blot is available for an additional fee. Ten wells (highest absorbency by ELISA) will be amplified and cloned for parental hybridomas. If after first fusion attempt no acceptable development is observed, a second fusion attempt will be made utilizing previously immunized mice remaining from Phase A.

PHASE C.
Cloning
(approximately an additional 8 weeks totaling about 22-26 weeks)
One to five of the ten previously developed parental hybridomas will be selected, cloned and then screened by ELISA. Clones with desired titer and specificity will be expanded, utilized for production and/or cryopreserved. Up to five clones (two subclones for each parental hybridoma line) will be provided and delivered to the customer depending on the stability of the chosen hybridomas. If additional clones are identified, they can be expanded for an additional charge.

PHASE D.
Monoclonal Antibody Production Production by In-Vitro Methods Only:
Tissue Culture Bags, Bioreactors and other methods can be used depending on your specific requirements. Please contact us for a quote as per your needs.