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Polyclonal Antibody Products
PRF&L Immunization
and Bleeding Protocols for Custom Polyclonal Antibody Production
Our protocols have been developed over our
45 years of being in the antibody production business. We have found these protocols to be generally
very successful. However, we cannot guarantee satisfactory results as all antigens are different, as are
all animals' immune systems. Our protocols are designed to give highest possible and sustained titers for
optimal antibody collection. Keep in mind that if you have a protocol that has worked well for you in the past,
feel free to request that we follow your protocol. We have followed a variety of immunization schedules
to meet the unique needs of our investigators. A particular protocol is not always the best for all antigens. PRF&L Standard Immunization
/ Bleeding Protocols by Species:
PREIMMUNE SCREENING PROCEDURE -
You may request that we take preimmune bleeds from a group of animals prior
to any injections so you can examine them
for background activity. This allows you to choose the animals with
the most desirable background for antibody production. This is especially important for yeast proteins in rabbits.
We charge you a flat
rate per animal plus shipping that
includes the bleed and up to 7 days of daily maintenance time
to evaluate the bleed and let us know which animals are desirable for antibody
production.
Please have your screening methodologies ready prior to requesting the prescreening
bleeds to avoid fees. We reserve the right to charge maintenance fees for animals not chosen within
seven days of receipt of serum. You may request any number of animals for prescreening, however,
we suggest three for every one animal you want to use for antibody
production. We only charge you setup fees for animals that you choose for
antibody production! Submit your request for this service by submitting a Client Information Form and
contact us with the species and number of animals you wish to screen.
