CUSTOM
ANTIBODY PRODUCTION
Our experience shows that this protocol works well for certain fusion protein antigens. Please note that the immunization schedule includes two intradermal injections and one intravenous injection.
For intravenous (IV) injections, the antigen preparation must be able to pass easily through a 23-gauge needle and must be formulated in PBS buffer.
Please note that this protocol is not suitable for antigens formulated in a gel.
84 - Day Fusion Protein Antigen Polyclonal Antibody Production in Rabbits
The 84 Day Fusion Protein Protocol has been a favorite for many years at Pocono Rabbit Farm & Laboratory. The protocol uses IV injection at Day 56 as a way to improve the antibody. The protocol includes Setup, Prebleed, six immunizations, two large immune bleeds, and maintenance. This Protocol can be extended if additional serum is required. Price is per animal, unless noted.
Day 0- Pre-Bleed/Immunization
Day 28- Immunization
Day 42 - Immunization
Day 56- Small Bleed/ Immunization
Day 63 - Large Bleed
Day 70 - Immunization
Day 84- Large Bleed or Final Bleed (Approximate volume of sera provided per animal for the duration of the protocol is 65-90 ml).
For Open Projects, the Client can choose one of the Following Extensions:
EXTENSION A: Boost and Bleed- Includes 1 Boost immunization, 1 additional bleed, and 28 days of additional maintenance.
EXTENSION B: Production-Includes 4 production bleeds, 1 boost immunizations, and 28 days of additional maintenance.
EXTENSION C: Hold - Animals are held pending your decision to proceed. No boosts or bleeds.
